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sgot_ast [2025/01/27 11:23] – created adminsgot_ast [2025/01/27 11:28] (current) – admin
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 +|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]|
  
- +                          SGOT (AST) Examination Procedure
  
 **1.Purpose of examination:**  **1.Purpose of examination:** 
-  * AST estimation from serum NADH  (without P-5’-P) Method.+AST estimation from serum NADH  (without P-5’-P) Method.
  
 **2.Responsibility and Authority:**  **2.Responsibility and Authority:** 
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   * Type of Sample: Serum,     * Type of Sample: Serum,  
   * Type of container and additives: Plain without any additives    * Type of container and additives: Plain without any additives 
-  * Patient Preparation: As per Primary Sample Collection Manual {{Sample collection manual}}+  * Patient Preparation: As per Primary Sample Collection Manual [[sample_collection_manual|]]
   * Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours   * Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours
   * Handling and transport: As per Primary Sample collection manual    * Handling and transport: As per Primary Sample collection manual 
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 **8.Quality control Procedure:** **8.Quality control Procedure:**
-**Name:** Bio Rad Level 1 & 2 + 
-**Frequency:** As per Quality Control Procedure+  * **Name:** Bio Rad Level 1 & 2 
 +  * **Frequency:** As per Quality Control Procedure
  
 **Procedure for Reconstitution of IQC**  **Procedure for Reconstitution of IQC** 
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   * Put respected carrier in RSH rack   * Put respected carrier in RSH rack
  
-11.Performance Characteristics: +**11.Performance Characteristics:** 
   * Linearity:  5 to 4001 U/L   * Linearity:  5 to 4001 U/L
   * The limit of detection (LOD): 4 U/L   * The limit of detection (LOD): 4 U/L
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   - Kaplan LA,Pesce AJ editors. Clinical Chemistry Theory, Analysis, and Correlation, 3rd ed.   - Kaplan LA,Pesce AJ editors. Clinical Chemistry Theory, Analysis, and Correlation, 3rd ed.
  
 +|**Printed copy of this document is considered uncontrolled.** It should be compared with controlled electronic copy before use|
  
 +^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^
 +^Document No.1^**Document Name**: SGOT (AST) Examination Procedure^**Unique ID**:LSSTH /BIOCHEM/ SOP-5^^
 +^Issue No. : 01^Issue Date :30/04/2024^Page No.^^
 +^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^