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 **4.Required Equipment:**  **4.Required Equipment:** 
-●Centrifuge, Auto-Pipette, Disposable Tips, Disposable sample cups, FullyAuto Chemistry Analyzer+Centrifuge, Auto-Pipette, Disposable Tips, Disposable sample cups, FullyAuto Chemistry Analyzer
  
-5.Required reagents:+**5.Required reagents:**
 R1: Glycyl glycine buffer (pH 8.6) 255 mmol/L R1: Glycyl glycine buffer (pH 8.6) 255 mmol/L
-Sodium bicarbonate 46 mmol/L +  * Sodium bicarbonate 46 mmol/L 
-Iron (II) chloride 20 μmol/L+  * Iron (II) chloride 20 μmol/L
 R2:Ascorbic acid (pH 2.5) 300 mmol/L R2:Ascorbic acid (pH 2.5) 300 mmol/L
-Ferene-S 12.5 mmol/L+  * Ferene-S 12.5 mmol/L
  
-6.Reagent Handling +**6.Reagent Handling** 
- ● Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles +  * Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles 
-Reagent Storage and stability +  * Reagent Storage and stability 
-Unopened reagent stable at 2-8°C until expiration date. +  * Unopened reagent stable at 2-8°C until expiration date. 
-On board System temperature reagent is  stable for 28 days. +  * On board System temperature reagent is  stable for 28 days. 
-Instability or deterioration should be suspected if there are precipitates, visible signs of leakage or +  * Instability or deterioration should be suspected if there are precipitates, visible signs of leakage or 
-contamination, turbidity, or if calibration or controls do not meet the appropriate criteria.+  * contamination, turbidity, or if calibration or controls do not meet the appropriate criteria.
  
 **7.Calibration Procedure:** **7.Calibration Procedure:**