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+++ sample_collection_manual [2025/01/25 05:53] (current) – admin
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+[[clinical_biochemistry_section|Home]]
               SAMPLE COLLECTION MANUAL OF LABORATORY SERVICES
                       SIR T HOSPITAL, BHAVNAGAR
@@ Line 546: / Line 548: @@
 |Receiving|*R1 will receive it. Check the demographics and sign the register.||*R1 will receive the slides, check requisition form and give pap number on case paper.|
 |Responsibility|Collection| Clinical Resident/Consultant| Clinical Resident/Consultant|
-||Transport Class IV of ward| Class IV of OPD/Patient’ relative|
+|              |Transport| Class IV of ward| Class IV of OPD/Patient’ relative|
-||Receiving R1 Pathology | R1 Pathology|
+|              |Receiving| R1 Pathology | R1 Pathology|
+                    ''STANDARD OPERATING PROCEDURES (LSSTH/SCM/SOP/RSH)1/1''
+**7.1 STANDARD OPERATING PROCEDURE OF RECEIVING ROUTINE SPECIMENS IN HISTOPATHOLOGY**
+  * The histopathology requisition form should be complete with following details -name of the patient, age, sex, requisition no., ward no., name of the incharge Dr, history or clinical diagnosis, nature of the specimen, date of operation.
+  * Specimen should be received only if sent in formalin.
+  * Note the date of receipt of specimen.
+  * After checking all the details give histopathology no. to jar.
+  * Ask history from patient’s relatives if that found on requisition slip is inadequate.
+  * Give receipt no. and date of issue of report to the patient.
+  * Formalin and container should be changed s o s if..
+  - if  foamy.
+  - Reddish.
+  - Top up with formalin if not 10times the volume of the tissue.
+  - If organ is too large for container or if container is cracked or not closing  properly.
-Fix immediately.
-Rou
-tine               	8:00 am to 4:00 pm Transfer within 1 hour. 	During OPD timing.
-Fix immediately.
-	Sat urd ay 	8:00am to 12:00 pm. Transfer within 1 hour.
-	Sun day
-&
-Holi	Avoid collection.If required to collect,
-aliquot and keep in
-ward refrigerator at 2-8 C and
+                 ''STANDARD OPERATING PROCEDURES (LSSTH/SCM/SOP/FSCP)1/1''
+**7.2 STANDARD OPERATING PROCEDURE OF FNAC SAMPLE COLLECTION PROCEDURE**
+  * Procedure is usually performed in sitting position in chair. For Thyroid lesion usually lying down position with pillow under the shoulder is preferred.
+  * Examine the swelling in terms of Location, Size, Shape, Consistency, Tenderness, Mobility and  Movement.
+  * Clear the area of swelling with skin disinfectant.
+  * Allow it to evaporate. Explain the procedure to the patient.
+  * Insert the needle (No. 23G) with 10 cc syringe within the target tissue.
+  * Pull the plunger to apply negative pressure.
+  * Move the needle back and forth inside the target tissue.
+  * Release the negative pressure while needle remains in target tissue after obtaining the sample in the hub.
+  * Withdraw the needle.
+  * Put dry cotton swab with gentle pressure over the puncture site.
+  * Detach the needle.
+  * Take out material from the needle on the clean dry glass slide.
+  * Make a smear by using another glass slide.
+  * Fix the smear immediately in methanol for H & E stain( for Giemsa stain-air dry smear and then fix).
+  * Write the identity number on the slides and Stain the smear.
+              ''STANDARD OPERATING PROCEDURES (LSSTH/SCM/SOP/SRC)1/5''
+**7.3 SAMPLE REJECTION CRITERIA BLOOD SAMPLE REJECTION CRITERIA**
+  * Improperly filled forms.
+  * Improperly labeled samples.
+  * Inappropriate quantity of sample.
+  * Clotted sample (in Pathology Section)
+  * Spill over sample.
+  * Hemolyzed sample.
+  * Sample received after 4 hours of collection.
+  * Diluted Sample
+  * Improper Vacutainers
+**CRITERIA FOR SAMPLE REJECTION IN HISTOPATHOLOGY**
+  * Grossly autolysed specimen
+  * Sample sent without 10/ formalin
+  * Container sent without tissue
+  * Quantity of formalin should be approx . 10 times of tissue
+  * Appropriate size of container
+  * Incomplete sample labeling
+  - Patient’s Name , Reg. no, Ward
+  - Specimen name
+**Incomplete requisition form**
+  - Patient’s Name, Reg. no, Ward/Unit
+  - Name of unit Incharge
+  - Related history and investigation
+  - Clinical diagnosis
+  - Specimen name
+  - Name and signature of authorized person
+**FNAC REJECTION CRITERIA**
+  * Incompletely filled up form.
+  * Smears not properly labeled.
+  * Smears are not properly spread.
+  * Smears containing clots.
+  * Smears (Material) diluted with blood.
+  * Smears not properly fixed.
+         ''STANDARD OPERATING PROCEDURES (LSSTH/SCM/SOP/SRC)2/5''
+**REJECTION CRITERIA OF MICROBIOLOGY SPECIMENS**
+**Purpose:** To provide Microbiology specimen rejection criteria & the procedure to follow when rejecting specimens for process.
+**General Principle:**
+Proper collection & transport of specimens is critical to the quality of results produced by the microbiology Laboratory & cannot be overemphasized. The validity of all diagnostic information produced in the lab is contingent on the quality of the specimen received. Consequences of poorly collected and/or poorly transported specimens include failure to isolate the causative organism, and recovery of contaminants or normal flora could lead to improper treatment of the patient. The saying is “garbage in—garbage out”.
+**General Consideration:**
+When a specimen is received in the laboratory, it is subjected to scrutiny & must meet certain minimum requirements before being accepted for testing.
+A specimen shall not be rejected until appropriate efforts have been made to correct the problem, but it may be rejected for failing to meet acceptability criteria.
+If, needed the specimen is rejected, it is listed on the microbiology disposition log for rejected specimens, along with reason for rejection & documentation of notified personnel.
+**Rejection criteria:**
+A. //SPECIMEN RECEIVED WITHOUT A LABEL OR WITH AN IMPROPER LABEL//
+  * At a minimum, specimen label must include patient name & reg. no. as the two patient identifiers.
+  * Call unit, ward or person responsible & notify of the problem.
+  * If any doubt exists as to origin of specimen, reject & order recollection.
+B.//SPECIMEN IMPROPERLY COLLECTED OR TRANSPORTED//
+  * Check with person responsible for collection to determine reason for deviation from procedure. Give instruction in the proper method of collection & transport to prevent repetition of the error.
+C.//SPECIMEN CONTAINER EXHIBITS GROSS EXTERNAL CONTAMINATION OR LEAKING SPECIMEN//
+  * Container exteriors must be clean & free of gross contamination. Leaking specimens indicate integrity of the specimen has been compromised & puts transport & laboratory personnel at risk.
+D.//SPECIMEN NOT OF SUFFICIENT QUANTITY//:If specimen has multiple orders, request priority listing of tests.
+E.//SPECIMEN MUST BE RECEIVED IN A TIMELY MANNER// Order recollection if specimen has exceeded recommended limits for transport.
+^Sr. No.^Specimen type^Test^Rejection Criteria^Report Comment^
+|1|Stool|C&S|1.Not submitted in enteric pathogen transport media. 2.Multiple specimens collected from the same in-patient the same day(only one specimen per patient per test per day is to be processed).3.All formed stools except when S. typhi requested.|1.Specimen not received in enteric transported medium.2.Multiple specimens received. Only the most recently collected specimen has been processed.3.Formed stool received. Test cancelled.|
+|2|Sputum|C&S|More than 25 squamous epithelial cells/lower power field|Greater than 25 squamous epithelial cells per low power field.|
+|3|Urine|C&S|  * Condom catheter  * Foley catheter tips & bags  * Leaking specimens  * Inappropriate/swabs/non sterile container  * > 24 hr delay before specimen received  * Duplicate specimens (more than one processed urine within 24 hrs)|“Specimen unsuitable for culture…. Because of….add qualifier.”|
+|4|Dry swabs|C&S|If received in the lab > 1 hr after collection.|“Specimen not received in transport medium & was in transit for > 1 hr.”|
+|5|Blood cultures, tissues, Sterile body fluids e.g. CSF|C&S|If received in the lab > 48 hr after collection.| Process specimen. EnterReport Comment:”Specimen was received in the lab>48 hrs after it has been collected; results should be interpreted accordingly.”|
+|6|All specimen type except: Tissue Sterile, body fluid,Brain & liver abscess, Aspirated pus, Bone & soft tissue biopsies with diagnosis of gas gangrene, necrotizing fasciitis or necrotizing cellulitis |Anaerobic culture| Not submitted in special anaerobic transport media.| “No Anaerobic swab received; anaerobic culture not done.”|
+|7|Blood | For serology|* Sample hemolysed.* Not submitted in Red vacuette| * So kindly repeat the sample.” * Please collect blood in Red vacuette.|
+^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^
+^**Document Name**: Documented procedure for primary sample collection & handling(Sample collection manual)^^^
+^**Unique ID**: LSSTH/B/Central/DP/7.2.4/22^^^
+^Issue No. : 01^Issue Date :30/04/2024^Copy No. :01^
+^Authorized by:Laboratory director^ Review Date:23/09/2024^Reviewed by:Deputy Quality Manager^
-STANDARD OPERATING PROCEDURES (LSSTH/SCM/SOP/RSH)1/1
-.1    STANDARD OPERATING PROCEDURE OF RECEIVING ROUTINE           SPECIMENS IN HISTOPATHOLOGY
-●	The histopathology requisition form should be complete with following details -name of the patient, age, sex, requisition no., ward no., name of the incharge Dr, history or clinical diagnosis, nature of the specimen, date of operation.
-●	Specimen should be received only if sent in formalin.
-●	Note the date of receipt of specimen.
-●	After checking all the details give histopathology no. to jar.
-●	Ask history from patient’s relatives if that found on requisition slip is inadequate.
-●	Give receipt no. and date of issue of report to the patient.
-●	Formalin and container should be changed s o s if..
--	if  foamy.
--	Reddish.
--	Top up with formalin if not 10times the volume of the tissue.
--	If organ is too large for container or if container is cracked or not closing  properly.