Differences

This shows you the differences between two versions of the page.

--- creatinine [2025/01/27 09:47] – admin
+++ creatinine [2025/01/27 10:54] (current) – admin
@@ Line 1: / Line 1: @@
-[[clinical_biochemistry_section|Home]]
+|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]|
-  * **Purpose of examination:**
+                       Creatinine Examination Procedure
+**1.Purpose of examination:**
   * Creatinine  estimation from serum or plasma by Kinetic Alkaline Picrate Method.
-  * **Responsibility and Authority:**
+**2.Responsibility and Authority:**
   * Calibration: Technician
   * Quality Control: Technician
@@ Line 11: / Line 12: @@
   * Overall Monitoring: Quality Manager
-  * **Sample Details:**
+**3.Sample Details:**
   - Type of Sample: Serum,  Plasma
   - Type of container and additives: Plain without any additives
@@ Line 19: / Line 20: @@
   - Storage: 24 hours at 2-8° C
-  * **Required Equipment:**
+**4.Required Equipment:**
   * Centrifuge, Auto-Pipette, Disposable Tips, Disposable sample cups, FullyAuto Chemistry Analyzer
+**5.Required reagents:**
-  * **Required reagents:**
   * R1. Sodium hydroxide 0.8 mol/L
   * R2. Picric acid 24 mmol/L
   * **Reagent Handling**
   * Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles
-**Reagent Storage and stability:**
+**6.Reagent Storage and stability:**
   * Unopened reagent stable at 15-30°C until expiration date.
   * On board System temperature reagent is  stable for 05 days
   * Instability or deterioration should be suspected if there are precipitates, visible signs of leakage or contamination, turbidity, or if calibration or controls do not meet the appropriate criteria.
-  * **Calibration Procedure:**
+**7.Calibration Procedure:**
   * Consolidated Chemistry  Calibrators(ConCC)
   * **Frequency:**
@@ Line 40: / Line 40: @@
   * Replacement in any parts of Instrument
-  * **Procedure:**
+**Procedure:**
   - Start the equipment.WDI abbotte fully.docx
   - Calibrators are ready to use.
@@ Line 50: / Line 50: @@
   - If control results fall outside acceptable ranges,root cause analysis or recalibration may be necessary.
-  * **Quality control Procedure:**
+**8.Quality control Procedure:**
 **Name:** Biorad Level 1 &2
@@ Line 56: / Line 56: @@
 **Frequency:** As per Quality Control Procedure
-  * **Procedure for Reconstitution of IQC**
+**Procedure for Reconstitution of IQC**
   - Reconstitution of QC material with 5 ml Distilled water by using calibrated fixed volume pipette.
   - Leave to stand for 30 min in the dark place.
@@ Line 65: / Line 65: @@
   - Prior to use, make sure that aliquots should be at room temperature for at least 15 min.
-  * **Procedure to run IQC**
+**Procedure to run IQC**
   - Press Control order
   - Select Assay /Panel, to be run.
@@ Line 74: / Line 74: @@
   - Check IQC results, in case outliers call residents.
-  * **Principle of the procedure used for examinations:**
+**9.Principle of the procedure used for examinations:**
 At an alkaline pH, creatinine in the sample reacts with picrate to form a creatinine-picrate complex. The rate of increase in absorbance at 500 nm due to the formation of this complex is directly proportional to the concentration of creatinine in the sample.
-  * **Sample Preparation:**
+**10.Sample Preparation:**
   * Required Sample Volume: 150 µl of the sample
   * Temperature: 37°
 Take 150-200µl of the sample from Primary tube to the secondary aliquot. Write the sample ID on the aliquot.
-  * **Procedure to run Patient sample**
+**Procedure to run Patient sample**
   - Press Patient order
   - Select Assay /Panel, to be run.
@@ Line 90: / Line 90: @@
   - Put respected carrier in RSH rack
-  * ** Performance Characteristics:**
+**11.Performance Characteristics:**
   * Linearity:   0.20 to 37.00 mg/dL
   * The limit of detection (LOD): 0.05 mg/dL
@@ Line 96: / Line 96: @@
   * Unit: mg/dl
-  * **Normal and critical ranges:**
+**12.Normal and critical ranges:**
 ^1-5 y^0.04-0.45^mg/dL^
@@ Line 103: / Line 103: @@
 ^Adult Female^0.45-0.75^mg/dL^
-  * **Laboratory Clinical interpretation:**
+**13.Laboratory Clinical interpretation:**
   * Measurement of serum creatinine is used to diagnose and monitor acute and chronic renal disease, estimate glomerular filtration rate (GFR), or assess the status of renal dialysis patients.
-  * **Interference and cross reaction:**
+**14.Interference and cross reaction:**
 The Following analytes were tested up to the levels indicated at Creatinine concentrations of 0.14mg/dl and 5.03 mg/dl, and found not to interfere:
   * No interference from Bilirubin up to 30 mg/dL
@@ Line 115: / Line 115: @@
   * No interference from Protein up to 10.6 g/dL
-  * **Potential source of variation:**
+**15.Potential source of variation:**
   * **Turn around time (TAT):**
   * Routine: 6.0 hours
   * Urgent: 2.0 hours
-  * **Recording of observation:**
+**16.Recording of observation:**
   * Software backup
   * Machine raw data
-**Storage & Disposal of waste:** Follow storage & discard procedure
+**17.Storage & Disposal of waste:** Follow storage & discard procedure
-  * **Environmental & Safety control:**
+**18.Environmental & Safety control:**
 **Reagent R1** contains sodium hydroxide that Causes severe skin burns and eye Damage & May be corrosive to metals
-* **Precautions:**
+**19.Precautions:**
   - Wear protective gloves / protective clothing / eye protection
   - Do not breathe mist / vapors / spray
   - Wash hands thoroughly after handling
-  - Keep only in original container**
+  - Keep only in original container
-  * **Response**
+**20.Response**
+  - IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
-  * IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
+  - IF IN EYES:Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing
-  * IF IN EYES:Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing
+  - IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water / shower.
-  * IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water / shower.
 **For Reagent R2:**
-* Explosive when dry
+  * Explosive when dry
-* Picric acid is a flammable solid when wet as a paste (i.e., not less than 10% water), and explosive when dry.
+  * Picric acid is a flammable solid when wet as a paste (i.e., not less than 10% water), and explosive when dry.
-* Prevent from forming crystals.
+  * Prevent from forming crystals.
-* Keep containers tightly sealed.
+  * Keep containers tightly sealed.
-* Do not allow to dry out.
+  * Do not allow to dry out.
+**21.References:**
+  - Thomas L, editor. Clinical Laboratory Diagnostics: Use and Assessment of Clinical Laboratory Results. Frankfurt, Germany: TH- Books Verlagsgesellschaft mbH; 1998:366–374.
+  -  Jaffe M. Ueber den Niederschlag, welchen Pikrinsaure in normalem Harn erzeugt und uber eine neue Reaction des Kreatinins. Hoppe Seylers Z Physiol Chem 1886;10:391–400.
+  - Folin O. Beitrag zur Chemie des Kreatinins und Kreatins Im Harne.Hoppe Seylers Z Physiol Chem 1904;41:223–242.
+  - Fabiny DL, Ertingshausen G. Automated reaction-rate method for determination of serum creatinine with the CentrifiChem. Clin Chem 1971;17:696–700.
+  - Soldin S, Henderson L, Hill G. The effect of bilirubin and ketones on reaction rate methods for the measurement of creatinine. Clin Biochem 1978:82–86.
+|**Printed copy of this document is considered uncontrolled.** It should be compared with controlled electronic copy before use|
- ** * References:**
+^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^
-Thomas L, editor. Clinical Laboratory Diagnostics: Use and Assessment of Clinical Laboratory Results. Frankfurt, Germany: TH- Books Verlagsgesellschaft mbH; 1998:366–374.
+^Document No.1^**Document Name**: Creatinine Examination Procedure^**Unique ID**:LSSTH /BIOCHEM/ SOP-5^^
- Jaffe M. Ueber den Niederschlag, welchen Pikrinsaure in normalem Harn erzeugt und uber eine neue Reaction des Kreatinins. Hoppe Seylers Z Physiol Chem 1886;10:391–400.
+^Issue No. : 01^Issue Date :30/04/2024^Page No.^^
-Folin O. Beitrag zur Chemie des Kreatinins und Kreatins Im Harne.Hoppe Seylers Z Physiol Chem 1904;41:223–242.
+^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^
-Fabiny DL, Ertingshausen G. Automated reaction-rate method for determination of serum creatinine with the CentrifiChem. Clin Chem 1971;17:696–700.
-Soldin S, Henderson L, Hill G. The effect of bilirubin and ketones on reaction rate methods for the measurement of creatinine. Clin Biochem 1978:82–86.