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--- ckmb [2025/01/29 04:47] – admin
+++ ckmb [2025/01/29 04:48] (current) – admin
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   * Check IQC results, in case outliers call residents.
-*9.Principle of the procedure used for examinations:**
+**9.Principle of the procedure used for examinations:**
-Creatine kinase catalyzes the reaction between creatine phosphate and ADP with formation of creatine and ATP. The ATP formed, in the presence of glucose and hexokinase (HK), yields ADP and glucose-6-phosphate. The glucose-6-phosphate formed in the presence of glucose-6-phosphate dehydrogenase (G6P-DH) reacts with β-NADP+ forming 6-phosphogluconate and β-NADPH. The presence of mouse antibodies that inhibit CK-MM activity in the reaction mixture allows the determination of the residual activity of CK-B isoenzymes (CK-MB
-and CK-BB). The CK-MB activity is obtained by multiplying the CK-B activity by two. By measuring the variation of the absorbance due to transformation of β-NADP+ into β-NADPH in a time interval at 340 nm,
+  * Creatine kinase catalyzes the reaction between creatine phosphate and ADP with formation of creatine and ATP. The ATP formed, in the presence of glucose and hexokinase (HK), yields ADP and glucose-6-phosphate. The glucose-6-phosphate formed in the presence of glucose-6-phosphate dehydrogenase (G6P-DH) reacts with β-NADP+ forming 6-phosphogluconate and β-NADPH. The presence of mouse antibodies that inhibit CK-MM activity in the reaction mixture allows the determination of the residual activity of CK-B isoenzymes (CK-MBand CK-BB). The CK-MB activity is obtained by multiplying the CK-B activity by two. By measuring the variation of the absorbance due to transformation of β-NADP+ into β-NADPH in a time interval at 340 nm,it is possible to calculate the residual activity in the examined sample.
-it is possible to calculate the residual activity in the examined sample.
 **10.Sample Preparation:**