Electrophoresis refers to the migration of charged solutes or particles of any size in a liquid medium under influence of an electrical field. Depending on the charge on the molecules, they move towards either anode or cathode.

Electrophoresis of positively charged particles (cations) is sometimes called cataphoresis, while electrophoresis of negatively charged particles (anions) is sometimes called anaphoresis.

Required reagent for protein electrophoresis

Hippurate buffer pH 8.8

1% Agarose gel

BPB(bromophenol blue) dye

Amido black stain

Methanol

5% glacial acetic acid

Steps of Serum protein electrophoresis

Gel preparation Clean the glass slide with tape water and and dry it with tissue paper. Cut a rectangle four borders from Xray plate and Stick Xray plate to periphery of glass slide so the thickness of gel is same as thickness of Xray plate.