Electrophoresis refers to the migration of charged solutes or particles of any size in a liquid medium under influence of an electrical field. Depending on the charge on the molecules, they move towards either anode or cathode.

Electrophoresis of positively charged particles (cations) is sometimes called cataphoresis, while electrophoresis of negatively charged particles (anions) is sometimes called anaphoresis.

=Required reagent=

Hippurate buffer pH 8.8

1% Agarose gel

BPB(bromophenol blue) dye

Amido black stain

Methanol

5% glacial acetic acid

1. Gel preparation Before applying the gel on the slide wash slide on which gel will be applied and dry it with tissue roll. Place slide on a levelled surface as shown in above figure. Stick microtap Stick microtap twice to the border of glass slide. so the thickness of gel is same as thickness of microtap layer.

TBE Buffer preparation 1. Tris base- 10.9 gm 2. Boric acid- 3.92gm 3. EDTA- 190 mg Dissolve all these substance in to 950 ml distilled water. Measure the pH of buffer. If it is less than 8.6 add NaOH crystal to make pH up to 8.6

buffer preparation

gel preparation

sample preparation