Theory Question, journal ,posting

Practical

1. Write type of transport mechanism and explain with example
2. Biochemical events occurring during skeletal muscle contraction.
3. Biochemical difference between muscle fibers and fuel used by sprinter and marathon runner
4. Cell membrane asymmetry
5. Structure and function of liposome, micelle and lipid bilayer.
6. Explain different type of protein structure with example. Explain primary structure relationship with protein function with two example. Write sequencing method to identify primary structure of protein.
7. EPA and DHA – biochemical basis of their role in health
8. Chaperones
9. Source and clinical significant of essential fatty acid
10. Collagen synthesis and its disorder
11. Write salvage pathway of purine and it’s disorder. Explain biochemical reason for different type of hyperurecemia and it’s management
12. Pathogenesis of Adenosine deaminase deficiency
13. Explain any five different mechanism of chemotherapeutic drug.
14. Write purine catabolism pathway and it’s disorder
15. Major features of human mitochondrial DNA
16. Write purine analogue
17. Biochemical reason of etiology and treatment of hyperuricemia
18. Type of Chromatography
19. Principle and instrumentation of nephelometry and turbidimetry
20. Principle of SDS PAGE for protein separation
21. Principle and utility of mass spectrometry
22. Principle, type and use of ELISA.
23. Draw diagram of a spectrophotometer. How double-beam-in-time spectrophotometer differ from double-beam-in-space spectrophotometer
24. Light sources in analytical equipments
25. Electrophoresis support media
26. Isoelectric focusing
27. Principle of SDS PAGE
28. Principle, instrumentation and uses of capillary electrophoresis
29. Affinity chromatography
30. Explain chromatographic resolution and efficiency
31. Quadrupole and linear ion trap mass analysers
32. Tandom mass spectrometry
33. Clinical applications of mass spectrometer
34. DNA motifs
35. Laboratory biosafety guideline related to covid 19
36. Write various analytic method with it’s principle, advantages, disadvantages, clinical interpretation of CRP, Ferritin, D-Dimer, IL-6 and procalcitonin.
37. Inflammatory markers of Covid-19
38. SARS CoV2 lifecycle
39. Mechanism of action of Vaccines against COVID19
40. Mechanism of action of Drugs against COVID19
41. Role of Clinical Biochemistry in COVID19 pandemic] - [[Inflammatory Markers in COVID19 management
42. Microteaching
43. OSPE and OSCE
44. Teaching methods for large group
45. Teaching methods for small group
46. Problem based learning
47. How to write good MCQ question paper
48. Potentiometry using Ion selective electrodes for H+, Na+, K+ and Cl-
49. Potentiometry electrodes for pCO2
50. Amperometric electrode for pO2
51. Amperometric O2 based and H2O2 based glucose electrodes
52. Mechanism of methotrexate and 5-flurouracil

1. Explain structure , type, variant , physiological role, synthesis and regulation of hemoglobin
2. Digestion and absorption of protein
3. Dietary fiber – It’s significant and biochemical reason
4. Regulation of enzyme activity.
5. Properties and significant of iso-enzyme.
6. Write type and it’s difference of diabetes mellitus. Explain biochemical changes occurs diabetesmellitus. Investigation require for diagnosis and prognostic evaluation of diabetic patients.
7. Metabolic changes in patient of diabetes mellitus ketoacidosis.
8. Explain any five example of competitive enzyme inhibition
9. Alpha-1 antitrypsin deficiency
10. HDL metabolism
11. Anti oxidant role of Vitamin
12. Protein targeting disorder.
13. Ketogenesis
14. Write significant of any five amino acid and explain it with related metabolic pathway.
15. Biochemical reason of hemolysis in G6PD deficiency and explain principle of Methelene Blue based screening test for diagnosis of it.
16. Regulation of cholesterol biosynthesis and explain of biochemical change of using statin group of drugs for short and long term
17. Differential diagnosis of jaundice on bases laboratory investigation.
18. Calculate energy formation of aerobic and anaerobic glycolysis
19. Pro-oxidant role of anti-oxidant.
20. Phase-II of xenobiotics.
21. Glycogen storage disease
22. Glycogenolysis is not the reverse of glycogenesis
23. Carnitine shuttle
24. Biochemical basis of etiology, clinical features, diagnosis and treatment of various Hyperhomocystinemia
25. Biochemical basis of etiology, diagnosis and treatment of unconjugated neonatal hyperbilirubinemia.
26. Amphibolic role of TCA cycle.
27. Metabolic changes during exercise.
28. Biochemistry basis of change in enzyme activity due to change in pH and temperature.
29. Biochemical explanation of galactosemia
30. Therapeutic role of enzyme
31. Fredrickson classification of hypercholesteremia
32. Explain molecular abnormality and detection technique for sickle cell anaemia
33. Vitamin K cycle and drugs acting on it.
34. Changes in various laboratory tests in iron deficiency anemia
35. Diagnostic role of enzyme
36. Lipid lowering drugs and it’s mechanism of action
37. Km and Vmax in competitive and noncompetitive enzyme inhibition
38. Cyanide poisoning – biochemical consequences and biochemical basis of its treatment
39. Uncouplers of respiratory chain
40. Thermogenesis in brown adipose tissue
41. Overview of carbohydrate metabolism
42. Overview of fatty acid metabolism
43. Overview of cholesterol metabolism
44. Overview of amino acid metabolism
45. Overview of transport of lipids in blood
46. HDL/VLDL/LDL/Chylomicron
47. Interconversion among glucose, TG and amino acids
48. Biochemical events in adipose tissue after a meal
49. Biochemical events in adipose tissue in fasting
50. Biochemical basis of etiology, clinical features, diagnosis and treatment of various hyperphenylalaninemia
51. Non-protein nitrogen products of tyrosine and tryptophan and various drugs affecting their metabolism
52. Maple syrup urine diseases
53. Biosynthesis and metabolism of serotonin and metatonin
54. Biochemical basis of etiology, clinical features, diagnosis and treatment of various hyperhomocystinemia
55. Biochemical basis of etiology, clinical features, diagnosis and treatment of various disorders of lipid malabsorption
56. Biochemical cause of major signs and symptoms of porphyrias
57. Biochemical basis of etiology, clinical features, diagnosis and treatment of unconjugated neonatal hyperbilirubinemia.
58. DM diagnostic guideline
59. ATP IV Hypercholesterolemia diagnostic guidline
60. beta thalassemia – genetics, pathogenesis, diagnosis

1. Write process of DNA replication and inhibition. DNA repair mechanism and it’s disorder
2. Type and effect of the mutation – explain each with example
3. Post-translational modifications in collagen
4. Point mutations and frame shift mutations
5. Oncogene and proto-oncogene
6. Genomic and cDNA library
7. Types of vaccines and it’s mechanism of action
8. Real time PCR
9. Viral integration and transposons in human genome
10. Structure, function and inhibitors of DNA topoisomerases
11. DNA damage – causes, types, repair pathways and repair fedality
12. Double stranded break repair mechanisms
13. Transcription cycle
14. Transcription control regions for eukaryotic genes
15. Post-trancriptional modification of primary transcript
16. Mechanism of intron removal and alternative splicing
17. Detection, quantification and identification of amplicon in real time PCR
18. Tumor marker
19. Restriction endonucleases
20. Structure, physiological Role and clinical importance of Inter Leukins
21. Enzymes used in recombinant DNA research
22. Immunoglobulin
23. Lac operon
24. Active and Passive immunity
25. Post-transcription modification of m-RNA.
26. Properties of genetic code
27. Write note somatic hyper mutation and iso-type switching of immunoglobin.
28. Regulation of m-RNA stability

1. Precaution and management of Needle stick injury
2. Biomedical Medical Waste management disposal rules
3. Extrinsic and Intrinsic coagulation pathway
4. Write specification for fully auto biochemistry analyser
5. 2012
6. Characteristic of good LIS
7. Format checklist on basis of objective structured practical examination (OSPE) to check technician’s competency for pipetting.
8. Explain biochemical basis of anti-inflammatory and anti-platelet action of aspirin, in context of it’s dose and duration of action.
9. Laboratory test to measure coagulation, thrombolysis and platelet aggregation.
10. Write blood and urine investigation require for differential diagnosis of renal failure and for evaluate prognosis of patient. Write biochemical reason for each parameter alteration. Write any three different estimation method with it’s principle of any these parameter.
11. LoB,LoD,LoQ and LoL
12. Newer advances in vacuum tubes technology for patient and phlebotomist safety
13. Concept of six sigma and measurement of sigma in a clinical chemistry laboratory
14. Calibrator traceability
15. Use of barcoding in clinical laboratory
16. Components of Integrated automation system in clinical laboratory
17. Advantages and disadvantages of POCT
18. Ideal requirements of POCT
19. Describe formation of creatinine in body Describe salient features of chemical and enzymatic methods for serum creatinine measurement. Give account of quality issues in creatinine measurement. List advantages and disadvantages of markers of GFR. Explain use of IDMS traceable MDRD equation for estimating GFR from serum creatinine.
20. Explain different types of isoenzymes and isoforms of Creatine Kinase in humans. Describe principle of measurement of CK-MB by catalytic method and mass method. Why the catalytic method generally contain N-acetyl cysteine, AMP and diadenosine-pentaphosphate.
21. Draw diagram of a spectrophotometer. How double-beam-in-time spectrophotometer differ from double-beam-in-space spectrophotometer
22. Amperometric O2 based and H2O2 based glucose electrodes
23. Desirable characteristics of EQA program for clinical chemistry
24. Quality indicators in NABL accredited clinical laboratory / requirements in ISO 15189-2012
25. Principle of SDS PAGE
26. Physiological role of liver.
27. Hormonal changes during menstrual cycle.
28. Absorption of iron
29. Root cause analysis in case of internal quality control outlier
30. Thyroid hormone synthesis
31. Pre-analytic variation
32. Training require for technical person working in clinical laboratory. Explain one in detail.
33. ABO blood groups
34. Type and Use of different blood collection tubes & order of draw for blood collection
35. Preparation require for apply for NABL accreditation
36. 2012
37. Methology for comparison sample result of analyte between different analytic methods and instruments.
38. Westgard rules for interpretation of QC results
39. Explain pre-analytic process for arterial blood gas analysis and it’s interpretation
40. Planning and technical requirement of internal audit of laboratory.
41. 2012 about documentation of examination procedures
42. Write policy/procedure to find new lot of internal quality control mean and sd for L-J chart.
43. Type of quality check method for biochemistry analyte.
44. Lactic acidosis – causes and biochemical basis of expected results of various laboratory examination
45. 2012 about Request form information
46. 2012 about verification and validation of examination procedure
47. Bland Altman plot for comparison between two methods
48. Name and function of fully automated biochemistry analyzer
49. Biochemical basis of expected results of various laboratory examination in renal tubular acidosis
50. Capillary blood sampling – methods and uses
51. Risk management in NABL accredited clinical laboratory / requirements in ISO 15189-2012
52. Recommended order of draw, stopper color, content and inversions
53. Types of vacuum tubes for collection of blood for serum
54. LJ Chart for quality control
55. Explain Trueness, accuracy and precision with examples
56. Explain repeatability and reproducibility with examples
57. Cholesterol reference material, method and laboratories
58. Structure, function and clinical co-relation of creatine kinase and it’s Isoenzymes.
59. Type of Chromatography
60. Principle and utility of mass spectrometry
61. Principle, type and use of ELISA.
62. Light sources in analytical equipments
63. Electrophoresis support media
64. Isoelectric focusing
65. Principle, instrumentation and uses of capillary electrophoresis
66. Affinity chromatography
67. Explain chromatographic resolution and efficiency
68. Quadrupole and linear ion trap mass analysers
69. Tandom mass spectrometry
70. Clinical applications of mass spectrometer
71. DNA motifs
72. Write various analytic method with it’s principle, advantages, disadvantages, clinical interpretation of CRP, Ferritin, D-Dimer, IL-6 and procalcitonin.
73. Potentiometry electrodes for pCO2
74. Amperometric electrode for pO2