**Fibrinogen estimation can not be done by serum**
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  * FIBRINOGEN HAS A MOLECULAR WEIGHT OF 3,40,000 D AND IS SYNTHESIZED BY THE LIVER.FIBRINOGEN LEVEL IN BLOOD IS 200-400 MG/DL
  * SERUM IS OBTAINED AFTER BLOOD CLOTTING, DURING WHICH FIBRINOGEN IS CONVERTED INTO FIBRIN AS PART OF THE CLOTTING PROCESS.
  * WHEN WE REMOVE BLOOD CLOTT WE ALSO REMOVE FIBRINOGEN IN THE CLOTT.
  * SO THAT , WE ARE NOT ABLE TO ESTIMATE FRIBRENOGEN FROM SERUM
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