Show pageOld revisionsBacklinksBack to top This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong. 1. Tris base- 10.9 gm 2. Boric acid- 3.92 gm 3. EDTA- 190 mg Dissolve all these substances in to 950 ml distilled water. Measure the pH of buffer. If it is less than 8.6 add NaOH crystal to make pH up to 8.6