Show pageOld revisionsBacklinksBack to top This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong. **Sample Preparation** Collect the blood sample in Red vacuette. Allow the sample to clot completely and centrifuge at 4000 rpm for 4 minutes. Take 200 microliter serum sample in to aliquot and add 20 microliter bromophenol blue dye and mix it.