Differences
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Next revision | Previous revision | ||
uibc [2025/01/29 04:56] – created admin | uibc [2025/01/29 04:57] (current) – admin | ||
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- Type of Sample: Serum, | - Type of Sample: Serum, | ||
- Type of container and additives: Plain without any additives | - Type of container and additives: Plain without any additives | ||
- | - Patient Preparation: | + | - Patient Preparation: |
- Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours | - Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours | ||
- Handling and transport: As per Primary Sample collection manual | - Handling and transport: As per Primary Sample collection manual | ||
Line 20: | Line 20: | ||
**4.Required Equipment: | **4.Required Equipment: | ||
- | ●Centrifuge, Auto-Pipette, | + | Centrifuge, Auto-Pipette, |
- | 5.Required reagents: | + | **5.Required reagents:** |
R1: Glycyl glycine buffer (pH 8.6) 255 mmol/L | R1: Glycyl glycine buffer (pH 8.6) 255 mmol/L | ||
- | Sodium bicarbonate 46 mmol/L | + | * Sodium bicarbonate 46 mmol/L |
- | Iron (II) chloride 20 μmol/L | + | |
R2:Ascorbic acid (pH 2.5) 300 mmol/L | R2:Ascorbic acid (pH 2.5) 300 mmol/L | ||
- | Ferene-S 12.5 mmol/L | + | * Ferene-S 12.5 mmol/L |
- | 6.Reagent Handling | + | **6.Reagent Handling** |
- | ● Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles | + | |
- | Reagent Storage and stability | + | |
- | Unopened reagent stable at 2-8°C until expiration date. | + | |
- | On board System temperature reagent is stable for 28 days. | + | |
- | Instability or deterioration should be suspected if there are precipitates, | + | |
- | contamination, | + | |
**7.Calibration Procedure: | **7.Calibration Procedure: |