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total_cholesterol [2025/01/28 04:44] – admintotal_cholesterol [2025/01/28 05:01] (current) – admin
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-                                                                                                                                                                                                                                                                                                                                                                                                                    +|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]| 
 + 
 +                      Total Cholesterol Examination Procedure                                                                                                                                                                                                                                                                                                                                                                                                                  
 **1.Purpose of Examination:**  **1.Purpose of Examination:** 
 To lay down standard operating procedure for serum Total Cholesterol estimation by CHOD-PAP Method in Biochemistry section of LSSTH, Bhavnagar. To lay down standard operating procedure for serum Total Cholesterol estimation by CHOD-PAP Method in Biochemistry section of LSSTH, Bhavnagar.
-**2.Principle of the procedure used for examinations: **  
-1)Cholesterol ester+H2O    2)Cholesterol+O2  
-                 ↓CHE                      ↓CHOD 
-Cholesterol+F.A               4-Cholestenon-3-one+H2o2 
  
-                    3)2H2O2+HBAl+4-Aminoantipyrine +**2.Principle of the procedure used for examinations: **  
-                                            ↓POD +The Cholesterol2 assay is an automated clinical chemistry assay. Cholesterol esters are enzymatically hydrolyzed by cholesterol esterase to cholesterol and free fatty acids. Free cholesterol, including that originally present, is then oxidized by cholesterol oxidase to cholest-4-ene-3-one and hydrogen peroxide. The hydrogen peroxide oxidatively couples with N,N-Bis(4-sulfobutyl)-3-methylaniline (TODB) and 4-aminoantipyrine to form a chromophore 
-                                   Quinoneimine dye+4H2O2 +(quinoneimine dye) which is quantitated at 604 nm.
-Which is quantitated at 500 nm.+
  
 **3.Performance:** **3.Performance:**
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 **4.Type of Primary Sample:** **4.Type of Primary Sample:**
-Serum or Plasma+Serum or Plasma [[sample_collection_manual|]]
  
 **5.Patient Preparation:** **5.Patient Preparation:**
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 **8.Enviromental & Safety control:** **8.Enviromental & Safety control:**
-Reagent R1 contains sodium azide.contact with acids liberates very toxic gas+Reagent R1 contains sodium azide. contact with acids liberates very toxic gas
  
 **9.Precautions:** **9.Precautions:**
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   * Automated Dilution Protocol   * Automated Dilution Protocol
   * When using the Automated Dilution Protocol, the system performs a dilution of the specimen and automatically corrects the concentration by multiplying the result by the appropriate dilution factor.   * When using the Automated Dilution Protocol, the system performs a dilution of the specimen and automatically corrects the concentration by multiplying the result by the appropriate dilution factor.
 +
 **17.Manual Dilution Procedure:** **17.Manual Dilution Procedure:**
   * Dilute the specimen with saline (0.85% to 0.90% NaCl).   * Dilute the specimen with saline (0.85% to 0.90% NaCl).
   * Enter the dilution factor in the Patient or Control order screen   * Enter the dilution factor in the Patient or Control order screen
 +
 **18.Stability of sample** **18.Stability of sample**
 Serum or plasma-At Room temperature18°–28°C (64°–82°F) stability ≤ 24 hours Serum or plasma-At Room temperature18°–28°C (64°–82°F) stability ≤ 24 hours
 +
 **19.Storage of sample** **19.Storage of sample**
- 2-8° C for 1 days (Retention period for re-examination and/or additional tests is 24 hrs.) + 2-8° C for 1 days (Retention period for re-examination and/or additional tests is 24 hrs.) 
 + 
 **20.Reagent Storage and stability**  **20.Reagent Storage and stability** 
 Reagent is stored at 2-8 °c.Reagent stability is 30 days if the reagent is uncapped and on board. Reagent is stored at 2-8 °c.Reagent stability is 30 days if the reagent is uncapped and on board.
 +
 **21.Turn around time (TAT)** **21.Turn around time (TAT)**
-  6.0 hours after collection,in case of emergency 2.0 hours.+6.0 hours after collection,in case of emergency 2.0 hours 
 **22.Critical Value:**NA **22.Critical Value:**NA
  
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   - NABL 112 effective from 01/06/2019   - NABL 112 effective from 01/06/2019
  
 +|**Printed copy of this document is considered uncontrolled.** It should be compared with controlled electronic copy before use|
  
 +^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^
 +^Document No.1^**Document Name**: Total Cholesterol Examination Procedure^**Unique ID**:LSSTH /BIOCHEM/ SOP-5^^
 +^Issue No. : 01^Issue Date :30/04/2024^Page No.^^
 +^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^