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 +|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]|
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 +                          SGPT (ALT) Examination Procedure
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 **1.Purpose of examination:**  **1.Purpose of examination:** 
   * ALT  estimation from serum or plasma by NADH (without Pyridoxal Phosphate) Method.   * ALT  estimation from serum or plasma by NADH (without Pyridoxal Phosphate) Method.
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   * Type of Sample: Serum,  Plasma   * Type of Sample: Serum,  Plasma
   * Type of container and additives: Plain without any additives    * Type of container and additives: Plain without any additives 
-  * Patient Preparation: As per Primary Sample Collection Manual {{Sample collection manual}}+  * Patient Preparation: As per Primary Sample Collection Manual[[sample_collection_manual|]]
   * Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours   * Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours
   * Handling and transport: As per Primary Sample collection manual    * Handling and transport: As per Primary Sample collection manual 
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 lactate dehydrogenase (5.000 KU/L) lactate dehydrogenase (5.000 KU/L)
 * R2. L-alanine  (89.090 g/L) * R2. L-alanine  (89.090 g/L)
-          α-ketoglutaric acid (13.150 g/L)+α-ketoglutaric acid (13.150 g/L)
 **6.Reagent Handling** **6.Reagent Handling**
   * Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles   * Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles
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 **12.Performance Characteristics:**  **12.Performance Characteristics:** 
-**Linearity:**  7 to 3271 U/L+  * **Linearity:**  7 to 3271 U/L
 Automated Dilution Protocol:The system performs a 1:5 dilution of the sample and automatically calculates the concentration by multiplying the result by the dilution factor. Automated Dilution Protocol:The system performs a 1:5 dilution of the sample and automatically calculates the concentration by multiplying the result by the dilution factor.
 **Manual Dilution Procedure:** **Manual Dilution Procedure:**
 Dilute the specimen with saline (0.85% to 0.90% NaCl). Enter the dilution factor in the Patient or Control order screen Dilute the specimen with saline (0.85% to 0.90% NaCl). Enter the dilution factor in the Patient or Control order screen
-**The limit of detection (LOD):** 4 U/L +  * **The limit of detection (LOD):** 4 U/L 
-**The limit of quantification(LOQ):**7 U/L +  * **The limit of quantification(LOQ):**7 U/L 
-**Unit:** U/L+  * **Unit:** U/L
  
 **Normal and critical ranges:** **Normal and critical ranges:**
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 **16.Recording of observation:** **16.Recording of observation:**
-Software backup  +  * Software backup  
-Machine raw data+  * Machine raw data 
 **17.Storage & Disposal of waste:** Follow storage & discard procedure **17.Storage & Disposal of waste:** Follow storage & discard procedure
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 **18.Environmental & Safety control:** **18.Environmental & Safety control:**
   * For in vitro diagnostic use   * For in vitro diagnostic use
   * Do not use component before the expiration date   * Do not use component before the expiration date
   * Do not mix material from different kit lot number.   * Do not mix material from different kit lot number.
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 **19.References:** **19.References:**
   - US department of labor, Occupational safety and health administration.   - US department of labor, Occupational safety and health administration.
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 +|**Printed copy of this document is considered uncontrolled.** It should be compared with controlled electronic copy before use|
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 +^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^
 +^Document No.1^**Document Name**: SGPT (ALT) Examination Procedure^**Unique ID**:LSSTH /BIOCHEM/ SOP-5^^
 +^Issue No. : 01^Issue Date :30/04/2024^Page No.^^
 +^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^