Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Next revision		 Previous revision
sgpt_alt [2025/01/27 11:08] – created adminsgpt_alt [2025/01/27 11:26] (current) – admin
Line 1: Line 1:
 +|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]|
 +
 +                          SGPT (ALT) Examination Procedure
 +
 **1.Purpose of examination:**  **1.Purpose of examination:** 
   * ALT  estimation from serum or plasma by NADH (without Pyridoxal Phosphate) Method.   * ALT  estimation from serum or plasma by NADH (without Pyridoxal Phosphate) Method.
Line 11: Line 15:
   * Type of Sample: Serum,  Plasma   * Type of Sample: Serum,  Plasma
   * Type of container and additives: Plain without any additives    * Type of container and additives: Plain without any additives 
-  * Patient Preparation: As per Primary Sample Collection Manual {{Sample collection manual}}+  * Patient Preparation: As per Primary Sample Collection Manual[[sample_collection_manual|]]
   * Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours   * Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours
   * Handling and transport: As per Primary Sample collection manual    * Handling and transport: As per Primary Sample collection manual 
Line 20: Line 24:
  
 **5.Required reagents:** **5.Required reagents:**
-  * R1.L-alanine (66.820 g/L) +* R1.L-alanine (66.820 g/L) 
-         β-NADH (0.305 g/L) +β-NADH (0.305 g/L) 
-         lactate dehydrogenase (5.000 KU/L) +lactate dehydrogenase (5.000 KU/L) 
-  * R2. L-alanine  (89.090 g/L) +* R2. L-alanine  (89.090 g/L) 
-          α-ketoglutaric acid (13.150 g/L)+α-ketoglutaric acid (13.150 g/L)
 **6.Reagent Handling** **6.Reagent Handling**
   * Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles   * Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles
Line 73: Line 77:
 ALT present in the sample catalyzes the transfer of the amino group from L-alanine to α-ketoglutarate, forming pyruvate and L-glutamate. Pyruvate in the presence of NADH and lactate dehydrogenase is reduced to L-lactate. In this reaction, NADH is oxidized to NAD+. The reaction is monitored by measuring the rate of decrease in ALT present in the sample catalyzes the transfer of the amino group from L-alanine to α-ketoglutarate, forming pyruvate and L-glutamate. Pyruvate in the presence of NADH and lactate dehydrogenase is reduced to L-lactate. In this reaction, NADH is oxidized to NAD+. The reaction is monitored by measuring the rate of decrease in
 absorbance at 340 nm due to the oxidation of NADH to NAD+. absorbance at 340 nm due to the oxidation of NADH to NAD+.
 +
 **10.Sample Preparation:** **10.Sample Preparation:**
   * Required SampleVolume: 150 µl of the sample    * Required SampleVolume: 150 µl of the sample 
   * Temperature: 37°    * Temperature: 37° 
   * Take 150-200µl of the sample from Primary tube to the secondary aliquot. Write the sample ID on the aliquot.    * Take 150-200µl of the sample from Primary tube to the secondary aliquot. Write the sample ID on the aliquot. 
 +
 **11.Procedure to run Patient sample** **11.Procedure to run Patient sample**
   - Press Patient order    - Press Patient order 
Line 85: Line 91:
  
 **12.Performance Characteristics:**  **12.Performance Characteristics:** 
-**Linearity:**  7 to 3271 U/L +  * **Linearity:**  7 to 3271 U/L 
-Automated Dilution Protocol: +Automated Dilution Protocol:The system performs a 1:5 dilution of the sample and automatically calculates the concentration by multiplying the result by the dilution factor.
-The system performs a 1:5 dilution of the sample and automatically calculates the concentration by multiplying the result by the dilution factor.+
 **Manual Dilution Procedure:** **Manual Dilution Procedure:**
 Dilute the specimen with saline (0.85% to 0.90% NaCl). Enter the dilution factor in the Patient or Control order screen Dilute the specimen with saline (0.85% to 0.90% NaCl). Enter the dilution factor in the Patient or Control order screen
-**The limit of detection (LOD):** 4 U/L +  * **The limit of detection (LOD):** 4 U/L 
-**The limit of quantification(LOQ):**7 U/L +  * **The limit of quantification(LOQ):**7 U/L 
-**Unit:** U/L+  * **Unit:** U/L
  
 **Normal and critical ranges:** **Normal and critical ranges:**
Line 115: Line 120:
  
 **16.Recording of observation:** **16.Recording of observation:**
-Software backup  +  * Software backup  
-Machine raw data+  * Machine raw data 
 **17.Storage & Disposal of waste:** Follow storage & discard procedure **17.Storage & Disposal of waste:** Follow storage & discard procedure
 +
 **18.Environmental & Safety control:** **18.Environmental & Safety control:**
   * For in vitro diagnostic use   * For in vitro diagnostic use
   * Do not use component before the expiration date   * Do not use component before the expiration date
   * Do not mix material from different kit lot number.   * Do not mix material from different kit lot number.
 +
 **19.References:** **19.References:**
   - US department of labor, Occupational safety and health administration.   - US department of labor, Occupational safety and health administration.
Line 129: Line 137:
  
  
 +|**Printed copy of this document is considered uncontrolled.** It should be compared with controlled electronic copy before use|
 +
 +^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^
 +^Document No.1^**Document Name**: SGPT (ALT) Examination Procedure^**Unique ID**:LSSTH /BIOCHEM/ SOP-5^^
 +^Issue No. : 01^Issue Date :30/04/2024^Page No.^^
 +^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^