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--- protein_electrophoresis [2023/06/21 11:13] – [principle] quality_manager
+++ protein_electrophoresis [2025/01/08 16:15] (current) – external edit 127.0.0.1
@@ Line 1: / Line 1: @@
 [[quality_manual|QM home]]
 ^Name^Unique ID^Edition^Date of Edition^
-|Documentary procedure for protein electrophoresis|LSSTH/A/QM/01| 1 | 01-03-2023|
+|Documentary procedure for protein electrophoresis|LSSTH/A/QM/01| 1 | 15-06-2023|
 ^Preparing authority^Approving authority^Review period^
@@ Line 52: / Line 52: @@
 **Sample Applicator Preparation**
 Prepare comb from the stainless steel as shown in image.
 Attach the magnetic strip to the ruler.
@@ Line 66: / Line 67: @@
   - Remove the applicator.
+**Running the samples in electrophoresis tank**
+  - Clean the electrophoresis tank with DI water and dry it with tissue paper before use.
+  - Put the electrophoresis tank on a table having even & leveled surface.
+  - Fill the cold buffer in the two compartment. Put the gel slide in electrophoresis chamber in such a way that sample applied should remain on cathodic side.
+  - Connect the gel with buffer through strip of filter paper wick & cover the chamber with its lid.
+  - Connect the electrode on the side of slide containing sample well to the cathode (-) & other electrode to the anode of the power supply.
+  - Set voltage at 300 volt and & run for 10 minutes.
+  - After 10 minutes,switch off the power supply & remove the slide.
+**Fixation**
+Put the slide in to methanol for 10 minutes for fixation so that proteins get denatured and remain at same position even after staining and destaining.
+**Drying of gel**
+Put the fixated glass slide in to hot air Owen at 50-60° C for 20 minutes.
+**Staining**
+Place the gel slide in to Amido black staining solution for 2 minutes.
+**Destaining**
+Remove the excess stain by putting the gel in to destaining solution for 15 minutes so that background becomes clear.
+**Interpretation of the  result**