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|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]| | |[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]| | ||
- | Sodium Examination Procedure | + | Sodium Examination Procedure |
- | 1.Purpose of Examination: | + | **1.Purpose of Examination: |
To lay down standard operating procedure for serum Sodium by ISE Method in Biochemistry section of LSSTH, Bhavnagar. | To lay down standard operating procedure for serum Sodium by ISE Method in Biochemistry section of LSSTH, Bhavnagar. | ||
- | 2.Principle: | + | **2.Principle: |
Ion Selective Electrode. | Ion Selective Electrode. | ||
- | 3.Performance: | + | **3.Performance: |
Linearity – 110-186 mEq/L | Linearity – 110-186 mEq/L | ||
Precision – It is determined from 2 runs per day with two replicates per run for 20 days on two AFT 300 Electrolyte Analyzer. | Precision – It is determined from 2 runs per day with two replicates per run for 20 days on two AFT 300 Electrolyte Analyzer. | ||
- | 4.Primary Sample: | + | **4.Primary Sample:** |
- | Plasma or serum | + | Plasma or serum [[sample_collection_manual|]] |
- | 5.Patient Preparation: | + | **5.Patient Preparation: |
Verbal consent of patient should be taken before collecting blood sample. | Verbal consent of patient should be taken before collecting blood sample. | ||
- | 6.Type of Container: | + | **6.Type of Container:** |
Plain Vaccute with no additive | Plain Vaccute with no additive | ||
- | 7.Required Equipment: | + | **7.Required Equipment:** |
Centrifuge, | Centrifuge, | ||
- | 8.Safety Precaution: | + | **8.Safety Precaution:** |
Follow the universal work precautions. | Follow the universal work precautions. | ||
- | 9.Calibration Procedure: | + | **9.Calibration Procedure:** |
Instrument performs auto calibration every four hours. | Instrument performs auto calibration every four hours. | ||
Manual calibration is done on following circumstances: | Manual calibration is done on following circumstances: | ||
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**21.Turn around time (TAT)** | **21.Turn around time (TAT)** | ||
- | | + | 6.0 hours after collection, in case of emergency 2.0 hours. |
**22.Potential source of variation: | **22.Potential source of variation: |