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glucose [2025/01/27 10:08] – adminglucose [2025/01/27 10:54] (current) – admin
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-                    Glucose Examination Procedure+|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]|
  
-**Purpose of examination:**  +                      Glucose Examination Procedure 
 + 
 +**1.Purpose of examination:**  
  Glucose estimation from serum or plasma by Hexokinase/G-6-PDH Method.  Glucose estimation from serum or plasma by Hexokinase/G-6-PDH Method.
  
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 Type of container and additives: Sodium fluoride/ Potassium oxalate vaccutte,glacial acetic acid container Type of container and additives: Sodium fluoride/ Potassium oxalate vaccutte,glacial acetic acid container
  
-Patient Preparation: As per Primary Sample Collection Manual {{Sample collection manual}}+Patient Preparation: As per Primary Sample Collection Manual [[sample_collection_manual|]]
  
 Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours
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   - Verify calibration with at least two levels of controls   - Verify calibration with at least two levels of controls
   - If control results fall outside acceptable ranges,root cause analysis or recalibration may be necessary.   - If control results fall outside acceptable ranges,root cause analysis or recalibration may be necessary.
- +**9. Quality control Procedure:**
-=== 10. Quality control Procedure: ===+
  
 1. Name: BioRad Level 1 & 2 1. Name: BioRad Level 1 & 2
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   - Check IQC results, in case outliers call residents.   - Check IQC results, in case outliers call residents.
  
-=== 11. Principle of the procedure used for examinations: ===+=== 10. Principle of the procedure used for examinations: ===
  
 Glucose is phosphorylated by hexokinase (HK) in the presence of adenosine triphosphate (ATP) and magnesium ions to produce glucose‐6-phosphate (G‐6-P) and adenosine diphosphate (ADP). Glucose-6-phosphate dehydrogenase (G-6-PDH) specifically oxidizes G-6-P to 6-phosphogluconate with the concurrent reduction of nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide reduced (NADH). One micromole of NADH is produced foreach micromole of glucose consumed. The NADH produced absorbs light at 340 nm and can be detected spectrophotometrically as an increased absorbance. Glucose is phosphorylated by hexokinase (HK) in the presence of adenosine triphosphate (ATP) and magnesium ions to produce glucose‐6-phosphate (G‐6-P) and adenosine diphosphate (ADP). Glucose-6-phosphate dehydrogenase (G-6-PDH) specifically oxidizes G-6-P to 6-phosphogluconate with the concurrent reduction of nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide reduced (NADH). One micromole of NADH is produced foreach micromole of glucose consumed. The NADH produced absorbs light at 340 nm and can be detected spectrophotometrically as an increased absorbance.
  
-=== 12. Sample Preparation: ===+===11.Sample Preparation: ===
  
 Required Sample Volume: 150 µl of the sample Required Sample Volume: 150 µl of the sample
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 Take 150-200µl of the sample from Primary tube to examination. Write the sample ID on the aliquot. Take 150-200µl of the sample from Primary tube to examination. Write the sample ID on the aliquot.
    
-=== 13. Procedure to run Patient sample ===+**12. Procedure to run Patient sample** 
  
   - Press Patient order    - Press Patient order 
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   - Put respected carrier in RSH rack   - Put respected carrier in RSH rack
  
-=== 14. Performance Characteristics: ===+**13. Performance Characteristics:** 
    
 1. Linearity:   05 to 800 mg/dL  1. Linearity:   05 to 800 mg/dL 
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-=== 14. Normal and critical ranges: ===+**14. Normal and critical ranges:** 
  
 ^Parameter^Biological Reference Interval^Prediabetes^Diabetes^ ^Parameter^Biological Reference Interval^Prediabetes^Diabetes^
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 ^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^ ^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^
-^Document No.1^**Document Name**: Glucose Examination Procedure^**Unique ID**: LSSTH/B/Bio/DP/7.2.2.b/27^^+^Document No.1^**Document Name**: Glucose Examination Procedure^**Unique ID**:LSSTH /BIOCHEM/ SOP-5^^
 ^Issue No. : 01^Issue Date :30/04/2024^Page No.^^ ^Issue No. : 01^Issue Date :30/04/2024^Page No.^^
 ^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^ ^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^