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| ckmb [2025/01/29 04:46] – created admin | ckmb [2025/01/29 04:48] (current) – admin | ||
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| - Type of Sample: Serum, | - Type of Sample: Serum, | ||
| - Type of container and additives: Plain without any additives | - Type of container and additives: Plain without any additives | ||
| - | - Patient Preparation: | + | - Patient Preparation: |
| - Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours | - Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours | ||
| - Handling and transport: As per Primary Sample collection manual | - Handling and transport: As per Primary Sample collection manual | ||
| Line 88: | Line 88: | ||
| * Check IQC results, in case outliers call residents. | * Check IQC results, in case outliers call residents. | ||
| - | *9.Principle of the procedure used for examinations: | + | **9.Principle of the procedure used for examinations: |
| - | Creatine kinase catalyzes the reaction between creatine phosphate and ADP with formation of creatine and ATP. The ATP formed, in the presence of glucose and hexokinase (HK), yields ADP and glucose-6-phosphate. The glucose-6-phosphate formed in the presence of glucose-6-phosphate dehydrogenase (G6P-DH) reacts with β-NADP+ forming 6-phosphogluconate and β-NADPH. The presence of mouse antibodies that inhibit CK-MM activity in the reaction mixture allows the determination of the residual activity of CK-B isoenzymes (CK-MB | + | |
| - | and CK-BB). The CK-MB activity is obtained by multiplying the CK-B activity by two. By measuring the variation of the absorbance due to transformation of β-NADP+ into β-NADPH in a time interval at 340 nm, | + | * Creatine kinase catalyzes the reaction between creatine phosphate and ADP with formation of creatine and ATP. The ATP formed, in the presence of glucose and hexokinase (HK), yields ADP and glucose-6-phosphate. The glucose-6-phosphate formed in the presence of glucose-6-phosphate dehydrogenase (G6P-DH) reacts with β-NADP+ forming 6-phosphogluconate and β-NADPH. The presence of mouse antibodies that inhibit CK-MM activity in the reaction mixture allows the determination of the residual activity of CK-B isoenzymes (CK-MBand CK-BB). The CK-MB activity is obtained by multiplying the CK-B activity by two. By measuring the variation of the absorbance due to transformation of β-NADP+ into β-NADPH in a time interval at 340 nm,it is possible to calculate the residual activity in the examined sample. |
| - | it is possible to calculate the residual activity in the examined sample. | + | |
| **10.Sample Preparation: | **10.Sample Preparation: | ||