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billirubin [2025/01/27 10:46] – created adminbillirubin [2025/01/27 11:12] (current) – admin
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 +|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]|
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 +                          Total Bilirubin Examination Procedure
 **1.Purpose of examination:**  **1.Purpose of examination:** 
   * Bilirubin Total estimation from serum or plasma by Diazonium Salt Method.   * Bilirubin Total estimation from serum or plasma by Diazonium Salt Method.
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   * Type of Sample: Serum,  Plasma   * Type of Sample: Serum,  Plasma
   * Type of container and additives: Plain without any additives    * Type of container and additives: Plain without any additives 
-  * Patient Preparation: As per Primary Sample Collection Manual {{Sample collection manual}}+  * Patient Preparation: As per Primary Sample Collection Manual [[sample_collection_manual|]]
   * Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours   * Stability: At Room temperature 18°–28°C (64°–82°F) stability ≤ 24 hours
   * Handling and transport: As per Primary Sample collection manual    * Handling and transport: As per Primary Sample collection manual 
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 **4.Required Equipment:**  **4.Required Equipment:** 
-●Centrifuge, Auto-Pipette, Disposable Tips, Disposable sample cups, FullyAuto Chemistry Analyzer+  * Centrifuge, Auto-Pipette, Disposable Tips, Disposable sample cups, FullyAuto Chemistry Analyzer
  
 **5.Required reagents:** **5.Required reagents:**
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 Hydrochloric acid 9.33 g/L  Hydrochloric acid 9.33 g/L 
   * R2. 2, 4-dichloroaniline 0.81 g/L   * R2. 2, 4-dichloroaniline 0.81 g/L
-            Hydrochloric acid  5.563 g/L +Hydrochloric acid  5.563 g/L 
-            Sodium nitrite   0.345 g/L +Sodium nitrite   0.345 g/L 
-             Surfactant     1.96%+Surfactant     1.96% 
 **6.Reagent Handling** **6.Reagent Handling**
   * Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles   * Remove any air bubbles present in the reagents with a new applicator stick, or allow the reagents to settle at the appropriate storage temperature to allow the bubbles to dissipate. To minimize volume depletion, do not use a transfer pipette to remove bubbles
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 ^1-2 d^6.0-10.0^mg/dL^ ^1-2 d^6.0-10.0^mg/dL^
 ^3-5 d^4.0-8.0^mg/dL^ ^3-5 d^4.0-8.0^mg/dL^
-^1 week -18 years^0-2.06mg/dL^+^1 week -18 years^0-2.06^mg/dL^
 ^Adult^0.2-1.5^mg/dL^ ^Adult^0.2-1.5^mg/dL^
  
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   * Software backup    * Software backup 
   * Machine raw data   * Machine raw data
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 **18.Storage & Disposal of waste:** Follow storage & discard procedure **18.Storage & Disposal of waste:** Follow storage & discard procedure
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 **19.Environmental & Safety control:** **19.Environmental & Safety control:**
 **Reagent R1** contains hydrochloric acid and sodium borohydride. Reagent R2 contains hydrochloric acid. **Reagent R1** contains hydrochloric acid and sodium borohydride. Reagent R2 contains hydrochloric acid.
 that Causes severe skin burns and eye damage. that Causes severe skin burns and eye damage.
-May be corrosive to metals+May be corrosive to metal 
 **20.Precautions:** **20.Precautions:**
   * Keep only in original container.   * Keep only in original container.
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   - ARCHITECT  TOTAL BILI 6L45 307147/R04   - ARCHITECT  TOTAL BILI 6L45 307147/R04
   - NABL 112 effective from 01/06/2019   - NABL 112 effective from 01/06/2019
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 +|**Printed copy of this document is considered uncontrolled.** It should be compared with controlled electronic copy before use|
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 +^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^
 +^Document No.1^**Document Name**: Total Bilirubin Examination Procedure^**Unique ID**:LSSTH /BIOCHEM/ SOP-5^^
 +^Issue No. : 01^Issue Date :30/04/2024^Page No.^^
 +^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^