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 +|[[clinical_biochemistry_section|Home]]|[[clinical_biochemistry|]]|[[examination_procedures|]]|
 +
 +                    Alkaline Phosphatase(ALP) Examination Procedure
 **1.Purpose of examination:**  **1.Purpose of examination:** 
 Alkaline Phosphatase estimation from serum or plasma by Para-nitrophenyl Phosphate Method. Alkaline Phosphatase estimation from serum or plasma by Para-nitrophenyl Phosphate Method.
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 **Name**: Biorad Level 1 & 2 **Name**: Biorad Level 1 & 2
 **Frequency:** As per Quality Control Procedure **Frequency:** As per Quality Control Procedure
 +
 **9.Procedure for Reconstitution of IQC**  **9.Procedure for Reconstitution of IQC** 
   - Reconstitution of QC material with 5 ml Distilled water by using calibrated fixed volume pipette.   - Reconstitution of QC material with 5 ml Distilled water by using calibrated fixed volume pipette.
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   * **Unit**: U/L   * **Unit**: U/L
  
- Normal and critical ranges:+**Normal and critical ranges:**
  
-^0-15 y^54-369^U/L^^ +^0-15 y^^54-369^U/L^ 
-^16-50 y^53-128^U/L^^+^16-50 y^^53-128^U/L^
 ^>50 y ^Male^56-119^U/L^ ^>50 y ^Male^56-119^U/L^
 ^>50 y ^Female^53-141^U/L^ ^>50 y ^Female^53-141^U/L^
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- Laboratory Clinical interpretation: +**14.Laboratory Clinical interpretation:** 
-● A rise in ALP activity occurs with all forms of cholestasis, particularly with obstructive jaundice. ALP is also elevated in disorders of the skeletal system that involve osteoblast hyperactivity and bone remodeling, such as Paget disease, rickets, osteomalacia, fractures, and malignant tumors. Moderate elevation of ALP may be seen in other disorders such as Hodgkin disease, congestive heart failure, ulcerative colitis, regional enteritis, and intra-abdominal bacterial infections.+  * A rise in ALP activity occurs with all forms of cholestasis, particularly with obstructive jaundice. ALP is also elevated in disorders of the skeletal system that involve osteoblast hyperactivity and bone remodeling, such as Paget disease, rickets, osteomalacia, fractures, and malignant tumors. Moderate elevation of ALP may be seen in other disorders such as Hodgkin disease, congestive heart failure, ulcerative colitis, regional enteritis, and intra-abdominal bacterial infections.
  
  
- Interference and cross reaction: +**15.Interference and cross reaction:** 
-No interference from Conjugated Bilirubin 15 up to  U/L +  * No interference from Conjugated Bilirubin 15 up to  U/L 
-No interference from Unconjugated Bilirubin 20 up to  U/L +  * No interference from Unconjugated Bilirubin 20 up to  U/L 
-No interference from Hemoglobin up to 250 U/L +  * No interference from Hemoglobin up to 250 U/L 
-No interference from Intralipid up to1500 U/L+  * No interference from Intralipid up to1500 U/L
  
-Potential source of variation: +**16.Potential source of variation:** 
 Turn around time (TAT): Turn around time (TAT):
 Routine: 6.0 hours Routine: 6.0 hours
 Urgent: 2.0 hours Urgent: 2.0 hours
  
- Recording of observation: +**17.Recording of observation:** 
-Software backup  +  * Software backup  
-Machine raw data +  * Machine raw data 
-Storage & Disposal of waste: Follow storage & discard procedure + 
-Environmental & Safety control: +**18.Storage & Disposal of waste:** Follow storage & discard procedure 
-Precautions: + 
-Wear protective gloves / protective clothing / eye protection +**19.Environmental & Safety control**: 
-Do not breathe mist / vapors / spray + 
-Wash hands thoroughly after handling +**20.Precautions:** 
-Keep only in original container +  * Wear protective gloves / protective clothing / eye protection 
-Response +  * Do not breathe mist / vapors / spray 
-IF SWALLOWED: Rinse mouth. Do NOT induce vomiting +  * Wash hands thoroughly after handling 
-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. +  * Keep only in original container 
-Continue rinsing +**21.Response** 
-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water / shower. +  * //IF SWALLOWED:// Rinse mouth. Do NOT induce vomiting 
-Reagent R2 contains methylisothiazolones that Causes severe skin burns and eye Damage . +  * //IF IN EYES:// Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing 
-Do not breathe mist / vapors / spray +  * //IF ON SKIN (or hair):// Take off immediately all contaminated clothing. Rinse skin with water / shower. 
-Wear protective gloves / protective clothing / eye protection +**Reagent R2** contains methylisothiazolones that Causes severe skin burns and eye Damage . 
-IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.+  * Do not breathe mist / vapors / spray 
 +  * Wear protective gloves / protective clothing / eye protection 
 + 
 +**22.References:** 
 +  - US department of labor,Occupational safety and health administration. 
 +  - US department of health and human services. Biosafety in MIcrobiological and biomedical laboratories. 
 +  - World health organization. Biosafety manual,3rd edition 
 +  - ARCHITECT  ALP 7D55 307217/R01 
 +  - NABL 112 effective from 01/06/2019 
 + 
 +|**Printed copy of this document is considered uncontrolled.** It should be compared with controlled electronic copy before use|
  
-References: +^ Name of Laboratory : Laboratory Services Sir T. Hospital (LSSTH),Bhavnagar ^^^^ 
-US department of labor,Occupational safety and health administration. +^Document No.1^**Document Name**: Alkaline Phosphatase(ALP) Examination Procedure^**Unique ID**:LSSTH /BIOCHEM/ SOP-5^^ 
-US department of health and human services. Biosafety in MIcrobiological and biomedical laboratories. +^Issue No. : 01^Issue Date :30/04/2024^Page No.^^ 
-World health organization. Biosafety manual,3rd edition +^Amend No.^ Amend Date ^Prepared by: Section Incharge^Approved & Issued by: HOD,Biochemistry^
-ARCHITECT  ALP 7D55 307217/R01 +
-NABL 112 effective from 01/06/2019+